ESAM anticorps

N° du produit ABIN204196

Cet anticorps Souris Monoclonal détecte spécifiquement ESAM dans WB, ELISA, ICC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour ESAM anticorps (ABIN204196)

Antigène: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ESAM est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-ESAM anticorps

Specificité: Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no cross-reactivity with rmESAM.

Purification: Protein G purified

Immunogène: NS0-derived rhESAM.

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA, Flo, ICC, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody. Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect ESAM in HUVECs. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Détails sur ESAM

Antigène: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Autre désignation: ESAM

Sujet: Name/Gene ID: ESAM

Synonyms: ESAM, LP4791 protein, W117m

ID gène: 90952

UniProt: Q96AP7